Biochemical changes during the development of witches’ broom: the most important disease of cocoa in Brazil caused by Crinipellis perniciosa. Scarpari LM(1). Crinipellis perniciosa is the cause of witches’ broom disease of cocoa, a serious problem in South America. The aim of the project was to develop a measure of. Pulse-field gel electrophoresis (PFGE) was used to determine the genome size and characterize karyotypic differences in isolates of the cacao biotype of.

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This will help to protect your privacy. Annals of Applied Biology, 89 2: Witches’ broom disease of cocoa in Rondonia, Brazil: A few minor primary and some secondary pathogens have been recorded on cocoa pods, with the most common of the latter group being Botryodiplodia theobromae.

All the branches, foliage and brooms should be cut up, making a compact layer of organic matter that will be quickly covered with fallen leaves and decompose rapidly. The material can then be spread on the ground so that it is all in contact with the leaf litter, as this speeds up decomposition.

Moniliophthora perniciosa (witches’ broom disease of cacao)

This saprotrophic mycelium can be readily isolated on agar media and produces degradative enzymes in vitro, but it cannot infect cocoa Rudgard et al. Brooms can remain productive for over 2 years. Productivity also varies on brooms of similar size taken from different clones.

Detailed description of symptoms of witches’ broom disease of cocoa caused by Crinipellis perniciosa.

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Open the calendar popup. Plant Pathology, 39 3: In cases where removal of individual brooms is feasible, vegetative brooms should be cut off at least cm below the point of infection.

However seeds and budwood should be grown, preferably in intermediate quarantine, for up to three growth flushes to allow expression of the disease from latent infections. The distribution of cocoa varies greatly across regions and countries, crinpellis almost continuous to sporadic.


Chlorophylls a and b were lower throughout the development of the disease while carotenoids and xanthophylls dropped in the infected tissue by the time of symptom development. Tropical Pest Management, 34 3: These factors also affect longevity and spore productivity of basidiomata, with most of them releasing spores over days if conditions perniiosa ideal, giving a total production of about 2. In other projects Wikimedia Commons. Hypocotyl broom Apparently healthy seeds from pods infected with M.

Crinipellis perniciosa

The discovery of a hyperparasitic fungus, Cladobotryum amazonense, on basidiomata of M. These results show co-ordinated biochemical alterations in the infected tissues, indicating major stress responses with the production of ethylene.

The Turrialba Method involves no appreciable yield loss if carefully managed. Description Top pernicosa page After Holliday, Contains Matches exactly Value is undefined. Cultural Control or Phytosanitation Sanitation is based on the concept that by removing infected plant parts the production of inoculum will cease, or at least be reduced to low levels, depending on the efficiency of the removal.

Sanctioned by Sanctioning name Validated by Type specimen or ex type More specimens Human pathogenicity code Plant pathogenicity code Code toxicity. The L-biotype, in contrast to its relatives exhibits a bifactorial outcrossing mechanism. First confirmed cronipellis of witches’ broom caused by Moniliophthora perniciosa on cacao, Theobroma cacao, in Saint Lucia.

These global losses will have increased as the disease continues to spread steadily through eastern Brazil Bahia State where it was first recorded in Almeida LC; de Andebrhan T, Less than 4 hours or more than 20 hours is inhibitory.

Meanwhile, a search for immune or resistant trees was made in South America in several expeditions, notably those of FJ Pound in the s. Many cocoa breeders have noted that not all trees in germplasm collections from wild cocoa populations are equally susceptible, and in earlier work advised the selection of the best bearing trees rather than those which appeared less susceptible, as the disease was considered controllable. Diseased material on cushions should be carefully removed by cutting it off as close as possible to the bark.


Prediction of any reductions in pod losses is more difficult for a sanitized field of cocoa surrounded by continuous plantations where brooms are not removed. Crinipellis perniciosa Stahel Singer, Crinipellis perniciosa var. In some cases more than one axillary bud may grow into a broom from the same original infection. Proof of pathogenicity of Crinipellis perniciosa to Theobroma cacao by using basidiospores produced in in vitro culture.

The age of the cocoa tissue at inoculation also influences infection. Host-Plant Resistance It is widely recognized that improvements in long-term control of witches’ broom can best be achieved by the use of resistant cocoa pernicios material, and the development of such material is urgently required.

Isolates of the fungus within this complex differ in both morphology and pathogenicity.

In plantations with canopies less than 3. Witches’ broom disease of cocoa a review. Successful phytosanitation is jeopardized when inoculum from adjacent or nearby farms reaches the sanitized area in sufficient quantities to cause appreciable pod loss and abundant infections on shoots and flower cushions.

These factors lead to loss or dilution of any active ingredient and result in the breakdown of disease control. The rehabilitation of old or heavily diseased plantings where cocoa has exceeded 5 m in height involves the application of treatments to increase the cocoa production of low-yielding trees.

Plantations of cocoa with the seedling habit may contain individuals that are exceptionally heavily attacked by witches’ broom, which may also be unproductive. Pathogenic variability amongst isolates of Crinipellis perniciosa from cocoa Theobroma cacao. Each method or combination of methods needs to be appraised in terms of cost and benefit, and against a background of fluctuating cocoa prices this presents other problems.

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